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Table 2 Overview of human recombinant IFNα2b production in prokaryotic and eukaryotic systems.

From: Stable high volumetric production of glycosylated human recombinant IFNalpha2b in HEK293 cells

Host mg/L Recovery Purity % Glycosylation Activity IU/mg* Ref.
   mg/L %     
Prokaryotic        
E. coli 5200 3000 58 ND No 3 × 109 [21]
E. coli 4000 300 7,5 ND No 2,5 × 108 [19]
E. coli 3500 600 12 100 No ND [20]
S. lividans 0,01 ND ND ND No 0,4 × 104 [55]
Eukaryotic        
Pichia pastoris 450 298 66,2 > 95 ND 1,9 × 109 [56]
Pichia pastoris 200 ND ND ND No 3.0 × 108 [57]
Tobacco BY2 cells 0.02 ND ND ND No ND [58]
Insect Sf9 cells ND ND ND ND Partial (no sialylation) 2,3 × 108 [34]
Mouse NS0 cells 120 ND ND ND Yes 2 × 108 [36]
  1. * IFNα2b activity has been determined by inhibition of viral replication. Different viruses and hosts were used.