Stable high volumetric production of glycosylated human recombinant IFNalpha2b in HEK293 cells

BMC Biotechnology

Table 2 Overview of human recombinant IFNα2b production in prokaryotic and eukaryotic systems.

Host	mg/L	Recovery		Purity %	Glycosylation	Activity IU/mg*	Ref.
		mg/L	%
Prokaryotic
E. coli	5200	3000	58	ND	No	3 × 10⁹	[21]
E. coli	4000	300	7,5	ND	No	2,5 × 10⁸	[19]
E. coli	3500	600	12	100	No	ND	[20]
S. lividans	0,01	ND	ND	ND	No	0,4 × 10⁴	[55]
Eukaryotic
Pichia pastoris	450	298	66,2	> 95	ND	1,9 × 10⁹	[56]
Pichia pastoris	200	ND	ND	ND	No	3.0 × 10⁸	[57]
Tobacco BY2 cells	0.02	ND	ND	ND	No	ND	[58]
Insect Sf9 cells	ND	ND	ND	ND	Partial (no sialylation)	2,3 × 10⁸	[34]
Mouse NS0 cells	120	ND	ND	ND	Yes	2 × 10⁸	[36]

* IFNα2b activity has been determined by inhibition of viral replication. Different viruses and hosts were used.

ISSN: 1472-6750