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Table 1 Quantification and recovery of HEK293-produced IFNα2b from two production schemes.

From: Stable high volumetric production of glycosylated human recombinant IFNalpha2b in HEK293 cells

Production scheme

IFNα2b mg/L (ELISA)

Percent recovery

 

Culture medium

SO3-column

 

1 feed

237 ± 11

185 ± 3

79.5

2 feeds

301 ± 25

216 ± 11

71.8