Figure 5
From: Stable high volumetric production of glycosylated human recombinant IFNalpha2b in HEK293 cells
HEK293-produced human IFNα2b is sialylated and O-glycosylated. IFNα2b was deglycosylated as described in material and methods. 1- 10 μg of purified HEK-produced IFNα2b. 2- 10 μg of purified HEK-produced IFNα2b digested with neuraminidase. 3- 10 μg of purified HEK-produced IFNα2b digested with O-glycosidase. 4- 10 μg of purified E. coli-produced IFNα2b.