Figure 1

Construction of the display library. cDNA fragments were cloned in Sfi1 sites of pAcTMVSVG vector providing N-terminal fusion with the gp64 signal peptide and C-terminal fusion with the transmembrane (TM) and cytoplasmic terminal domains (CTD) of the VSV G protein. The chimeric genes were expressed under the control of polyhedrin promoter and terminator. A, Schematic representation of baculovirus vector pAcTMVSVG. B, Details of the cloning site with, in bold, the start and stop sequences from gp64 and VSV G respectively, in lower case, the sequences of the vector around the Sfi1 cloning site, in vertical-orientation, the restriction sites in the multiple cloning site.