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Figure 4 | BMC Biotechnology

Figure 4

From: Dual-In/Out strategy for genes integration into bacterial chromosome: a novel approach to step-by-step construction of plasmid-less marker-less recombinant E. coli strains with predesigned genome structure

Figure 4

The cat -gene expression in MG- cat -(i)-strains. The dependence of the expression level of the cat-carrier cassetes (T7A2-cat) on their positions in the chromosome is indicated by arrows. Cat-activity of the MG-cat-(6) strain in which Cassette (T7A2-cat) is located in native φ80-attB was taken as 100%.

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