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Figure 5 | BMC Biotechnology

Figure 5

From: A comparative study of protein synthesis in in vitro systems: from the prokaryotic reconstituted to the eukaryotic extract-based

Figure 5

Analysis of rabbit reticulocyte lysate by size separation and western blotting. Top, SDS-PAGE analysis of the peak fractions (9 to 18) from the size-exclusion separation of a crude rabbit reticulocyte lysate. The molecular weight standards for the SDS-PAGE are indicated on the left. Based on the molecular weight standards for the size exclusion column, fractions 9–11 are >700 kDa; fractions 12–13 are 600–200 kDa; fractions 14–20 are 200–10 kDa. Bottom, western blot analyses of these fractions (9–18) using antibodies against Hsp70/Hsc70, Hsp40, Hsp90a and TCP-1a.

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BMC Biotechnology

ISSN: 1472-6750

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