Critical evaluation of HPV16 gene copy number quantification by SYBR green PCR

BMC Biotechnology

Table 1 Primers used to generate NA6 and for qPCR.

Primers	Sequence (5' to 3')	Product length (bp)	Reference
E2 F	AAC GAA GTA TCC TCT CCT GAA ATT ATT AG	81	[13]
E2 R	CCA AGG CGA CGG CTT TG
E6 F	GAG AAC TGC AAT GTT TCA GGA CC	80	[13]
E6 R	TGT ATA GTT GTT TGC AGC TCT GTG C
HMBS F	GCC TGC AGT TTG AAA TCA GTG	118	[32]
HMBS R	CGG GAC GGG CTT TAG CTA

ISSN: 1472-6750