Table 2 The proportion of neuronal and glial cells obtained in different culture conditions
Differentiation condition NHA-passage 0; 5–7 days | DMEM F12 with N2 | DMEM F12 with B27 | ||||||
|---|---|---|---|---|---|---|---|---|
- | bFGF | SHH | GDNF | - | bFGF | SHH | GDNF | |
Neuronal cells MAP2+ GFAP- CD44- | 50% | 56% | 54% | 57% | 50% | 54% | 55% | 55% |
Glial cells GFAP+, CD44+, MAP2- | 46% | 41% | 44% | 42% | 47% | 44% | 44% | 43% |
Undifferentiated cells MAP2+, GFAP+, CD44- | 4% | 3% | 2% | 2% | 3% | 2% | 3% | 2% |