Skip to main content
Figure 4 | BMC Biotechnology

Figure 4

From: Successful elimination of non-neural cells and unachievable elimination of glial cells by means of commonly used cell culture manipulations during differentiation of GFAP and SOX2 positive neural progenitors (NHA) to neuronal cells

Figure 4

Three derivatives of NHA cells present distinct expression of MAP2, GFAP and CD44. Results of triple immunostainings applied to derivatives of NHA cells obtained according particular differentiation protocols reveal: formation of one phenotypically homogenous [CD44+, MAP2-, GFAP-] non-neural population as a result of protocol 1 (a, b, c, d); presence of neuronal [MAP2+, CD44-, GFAP-] and glial [GFAP+, CD44+, MAP2-] populations in case of protocol 2 (e, f, g, h) and appearance of the third population of non-neural cells [CD44+, MAP2-, GFAP-] coexisting with neuronal [MAP2+, CD44-, GFAP-] and glial [GFAP+, CD44+, MAP2-] lineage in case of protocol 3 (i, j, k, l).

Back to article page

BMC Biotechnology

ISSN: 1472-6750

Contact us