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Figure 3 | BMC Biotechnology

Figure 3

From: Successful elimination of non-neural cells and unachievable elimination of glial cells by means of commonly used cell culture manipulations during differentiation of GFAP and SOX2 positive neural progenitors (NHA) to neuronal cells

Figure 3

Neural and non-neural differentiation of NHA cells resulted in changes in MAP2 and GFAP expression. Immunocytochemistry data based on double staining show: silencing of neural markers [MAP2, GFAP] in non-neural cells obtained according to protocol 1 (a, b, c); presence of equal proportion of neuronal [MAP2+, GFAP-] and glial [GFAP+, MAP2-] cells after differentiation of NHA cells according to protocol 2 (d, e, f) and presence of the third non-neural population of MAP2 and GFAP negative cells accompanying neuronal [MAP2+, GFAP-] and glial [GFAP+, MAP2-] population as a result of protocol 3 (g, h, i).

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BMC Biotechnology

ISSN: 1472-6750

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