Figure 5
From: Three-dimensional culture of human meniscal cells: Extracellular matrix and proteoglycan production
Immunolocalization of major ECM components in control and TGF-β-treated meniscal cells in 3D culture. The top row presents images from control cells; the second row presents images for TGF-β-treated cells. Chondroitin sulfate (CS): A: control cells show modest CS content in the ECM. B: ECM formed by TGF-β-treated cells shows more abundant CS compared to controls. Type I collagen (Col type I) is abundant in both ECM formed by control (C) and TGF-β-treated cells (D). Type II collagen (Col type II): Control cells produced less type II collagen (E) when compared to ECM from TGF-β-treated cells (F). Figure 5G presents a representative negative control specimen. (For all images, bar in G = 50 μm).