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Table 2 Summary of the recombinant constructs used for Agrobacterium-mediated transient and transgenic expression.

From: Expression of HIV-1 antigens in plants as potential subunit vaccines

Vector

Subcellular Target

Insert

Clone

pTRAc

cytosol

p24

pTRA-Cp24

  

p17/p24

pTRA-Cp17/p24-M

   

pTRA-Cp17/p24-NM

  

gag

pTRA-Cgag

pTRAkc-ERH

ER

p24

pTRA-ERp24

  

p17/p24

pTRA-ERp17/p24-M

   

pTRA-ERp17/p24-NM

  

gag

pTRA-ERgag

pTRAkc-rbcS1-cTP

chloroplast

p24

pTRA-CPTp24

  

p17/p24

pTRA-CPTp17/p24-M

   

pTRA-CPTp17/p24-NM

  

gag

pTRA-CPTgag

  1. All genes were Nicotiana spp. codon-optimised
  2. M = myristylated; NM = non-myristylated