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Figure 3 | BMC Biotechnology

Figure 3

From: Expression of HIV-1 antigens in plants as potential subunit vaccines

Figure 3

Western blot analysis of HIV p17/24 from infiltrated N. benthamiana leaves and fractionation of purified protein. Clarified sap samples were electrophoresed on a 12% SDS-PA gel and blotted onto nylon membrane. The membrane was probed with a rabbit polyclonal anti-p24. M, molecular weight marker; Gag, HIV B Gag reference sample; C, extract from leaves infiltrated with infiltration buffer; ER1 and ER2, extracts from two pTRA-ERp17/p24-M infiltrated leaves; CPT1 and CPT2, extracts from two pTRA-CPTp17/p24-M infiltrated leaves.

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