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Figure 4 | BMC Biotechnology

Figure 4

From: Human IL-12 p40 as a reporter gene for high-throughput screening of engineered mouse embryonic stem cells

Figure 4

Comparison of EGFP and IL-12 expression in the COS-7 cells transfected with pCMV-EGFP-IRES-p40, pPGK-EGFP-IRES-p40, and pRosa-EGFP-IRES-p40. a) schematic diagram of pCMV-EGFP-IRES-p40, pPGK-EGFP-IRES-p40, and pRosa-EGFP-IRES-p40 plasmids. B) The expression of EGFP and IL-12-p40 from the indicated plasmid were determined. EGFP mean fluorescence intensity (MFI) was measured by flow cytometry, and IL-12 p40 expression was assayed by ELISA. Values of EGFP and IL-12 p40 expression shown are the average of three plates transfected in parallel.

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