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Figure 3 | BMC Biotechnology

Figure 3

From: Human IL-12 p40 as a reporter gene for high-throughput screening of engineered mouse embryonic stem cells

Figure 3

Analysis of the CMV and PGK promoters in BHK-21, and COS-7 cells. a) Structures of the CMV-p40, PGK-p40, CMV-EGFP, and PGK-EGFP constructs. b) Transient EGFP and Il-12 p40 expression in transfected SHK and COS-7. EGFP mean fluorescence intensity (MFI) was measured by flow flow cytometry, and IL-12 p40 expression was assayed by ELISA. Values of EGFP and IL-12 p40 expression shown are the average of three plates transfected in parallel.

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BMC Biotechnology

ISSN: 1472-6750

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