Figure 1From: Human IL-12 p40 as a reporter gene for high-throughput screening of engineered mouse embryonic stem cellsTime course of IL-12 p40 activity changes in transfected cells. COS-7 and BHK-21 cell lines were transfetced with 100 ng each of PGK-p40 as described in MATERIALS AND METHODS. Supernatant media was collected 6, 9, 12, 24, 48 and 72 hours after transfecion and the IL-12 expression was measured for each time point by ELISA. Values of IL-12 p40 expression shown are the average of three plates transfected in parallel.Back to article page