Construction of a series of vectors for high throughput cloning and expression screening of membrane proteins from Mycobacterium tuberculosis

Table 2 Second round of expression screening as fusion proteins.

ORF	M.W. (kDa)	Fusion partner	cloned	IB*	M
Rv0011c	10.2	GST	Yes	XX**
Rv0460	8.1	GST	Yes	XX
Rv0544c	9.7	GST	Yes	X
Rv0882	9.6	GST	Yes	X
Rv1171	15.2	GST	Yes
Rv2128	7.4	GST	Yes	X
Rv3078	14.1	GST	Yes
Rv3632	13.1	GST	Yes
Rv3656c	7.1	GST	Yes	XXX
Rv3901c	15.4	GST	Yes	XXX
Rv0011c	10.2	MBP	Yes	XXX	XXX
Rv0460	8.1	MBP	Yes	XX	XX
Rv0544c	9.7	MBP	Yes	XX	XXX
Rv0882	9.6	MBP	Yes	X	XXX
Rv1171	15.2	MBP	Yes	X	X
Rv2128	7.4	MBP	Yes	XXX	XXX
Rv3078	14.1	MBP	Yes
Rv3632	13.1	MBP	Yes	X	X
Rv3656c	7.1	MBP	Yes	XXX
Rv3901c	15.4	MBP	Yes	XX	XXX
Rv0011c	10.2	KSI	Yes	XX
Rv0460	8.1	KSI	Yes	X
Rv0544c	9.7	KSI	Yes
Rv0882	9.6	KSI	Yes
Rv1171	15.2	KSI	Yes	X
Rv2128	7.4	KSI	Yes
Rv3078	14.1	KSI	Yes	X
Rv3632	13.1	KSI	Yes	X
Rv3656c	7.1	KSI	Yes	X
Rv3901c	15.4	KSI	Yes

* IB: Inclusion body fraction; S: Soluble fraction; M: Membrane fraction.
** Symbol "X" indicates the estimated yield of the fusion protein from Coomassie blue stained SDS-PAGE gel. One "X" corresponds to the expression yield between 10 mg and 20 mg for the fusion protein from 1 L LB culture.

ISSN: 1472-6750