Skip to main content

Table 1 Cloning and first round of expression screening of the targeted membrane proteins.

From: Construction of a series of vectors for high throughput cloning and expression screening of membrane proteins from Mycobacterium tuberculosis

ORF MW (kDa) # of TM Cloned Expression location * Expression Detection** Expression Level ***
Rv0007 31 2 Yes IB/M w/w ++
Rv0008c 15.7 1 Yes IB/M c/c +++
Rv0010c 15.2 2 Yes IB/M c/w +++
Rv0011c 10.2 2 Yes No   
Rv0012 28.3 1 Yes IB/M c/w ++++
Rv0150c 9.6 2 Yes IB c ++++
Rv0258c 16 2 Yes IB/M c/c ++++
Rv0345 14 2 Yes IB c ++++
Rv0420c 15.1 2 Yes IB c ++++
Rv0424c 10 1 Yes IB/M c/c ++++
Rv0426c 13.9 2 No    
Rv0460 8.1 3 Yes IB w ++
Rv0513 19.4 2 Yes IB/M w/w +
Rv0514 10.3 2 Yes IB w +
Rv0531 11.4 2 Yes IB/M w/w ++
Rv0544c 9.7 2 Yes No   
Rv0882 9.6 3 Yes No   
Rv1171 15.2 4 Yes IB/M w/w ++
Rv1214c 10.8 1 Yes IB c ++++
Rv1303 16.9 4 Yes IB/M c/c +++
Rv1382 18.2 2 Yes IB/M w/w ++
Rv1567c 10.4 2 Yes IB/M c/c +++
Rv1761c 13.5 1 Yes IB/M c/c ++++
Rv1772 10.9 2 Yes No   
Rv1811 24.8 4 Yes M w +
Rv2044c 11.9 3 Yes IB/M w/w ++
Rv2076c 9 2 Yes IB/M w/w ++
Rv2081c 14.2 1 Yes IB/M c/c +++
Rv2128 7.4 2 Yes No   
Rv2390c 19.9 1 Yes IB/M w/w ++
Rv2551c 13.8 4 Yes No   
Rv2654c 7.7 2 No    
Rv2668 18.3 2 Yes IB c +++
Rv2828c 19.6 2 Yes IB/M c/c ++++
Rv2843 17.7 3 Yes IB c ++++
Rv3078 14.1 4 Yes No   
Rv3346c 8.9 2 Yes No   
Rv3486 16 3 Yes IB/M w/w ++
Rv3632 13.1 3 Yes No   
Rv3656c 7.1 1 Yes No   
Rv3901c 15.4 1 Yes IB/M w/w ++
  1. * IB: Inclusion body fraction; M: Membrane fraction.
  2. ** Protein expression was detected by Coomassie blue stain (c) or Western blot (w) after SDS PAGE.
  3. *** Estimated expressed level: + < 0.5 mg/L; ++: 0.5–2 mg/L; +++: 2–10 mg/L; ++++: > 10 mg/L.