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Table 1 Cloning and first round of expression screening of the targeted membrane proteins.

From: Construction of a series of vectors for high throughput cloning and expression screening of membrane proteins from Mycobacterium tuberculosis

ORF

MW (kDa)

# of TM

Cloned

Expression location *

Expression Detection**

Expression Level ***

Rv0007

31

2

Yes

IB/M

w/w

++

Rv0008c

15.7

1

Yes

IB/M

c/c

+++

Rv0010c

15.2

2

Yes

IB/M

c/w

+++

Rv0011c

10.2

2

Yes

No

  

Rv0012

28.3

1

Yes

IB/M

c/w

++++

Rv0150c

9.6

2

Yes

IB

c

++++

Rv0258c

16

2

Yes

IB/M

c/c

++++

Rv0345

14

2

Yes

IB

c

++++

Rv0420c

15.1

2

Yes

IB

c

++++

Rv0424c

10

1

Yes

IB/M

c/c

++++

Rv0426c

13.9

2

No

   

Rv0460

8.1

3

Yes

IB

w

++

Rv0513

19.4

2

Yes

IB/M

w/w

+

Rv0514

10.3

2

Yes

IB

w

+

Rv0531

11.4

2

Yes

IB/M

w/w

++

Rv0544c

9.7

2

Yes

No

  

Rv0882

9.6

3

Yes

No

  

Rv1171

15.2

4

Yes

IB/M

w/w

++

Rv1214c

10.8

1

Yes

IB

c

++++

Rv1303

16.9

4

Yes

IB/M

c/c

+++

Rv1382

18.2

2

Yes

IB/M

w/w

++

Rv1567c

10.4

2

Yes

IB/M

c/c

+++

Rv1761c

13.5

1

Yes

IB/M

c/c

++++

Rv1772

10.9

2

Yes

No

  

Rv1811

24.8

4

Yes

M

w

+

Rv2044c

11.9

3

Yes

IB/M

w/w

++

Rv2076c

9

2

Yes

IB/M

w/w

++

Rv2081c

14.2

1

Yes

IB/M

c/c

+++

Rv2128

7.4

2

Yes

No

  

Rv2390c

19.9

1

Yes

IB/M

w/w

++

Rv2551c

13.8

4

Yes

No

  

Rv2654c

7.7

2

No

   

Rv2668

18.3

2

Yes

IB

c

+++

Rv2828c

19.6

2

Yes

IB/M

c/c

++++

Rv2843

17.7

3

Yes

IB

c

++++

Rv3078

14.1

4

Yes

No

  

Rv3346c

8.9

2

Yes

No

  

Rv3486

16

3

Yes

IB/M

w/w

++

Rv3632

13.1

3

Yes

No

  

Rv3656c

7.1

1

Yes

No

  

Rv3901c

15.4

1

Yes

IB/M

w/w

++

  1. * IB: Inclusion body fraction; M: Membrane fraction.
  2. ** Protein expression was detected by Coomassie blue stain (c) or Western blot (w) after SDS PAGE.
  3. *** Estimated expressed level: + < 0.5 mg/L; ++: 0.5–2 mg/L; +++: 2–10 mg/L; ++++: > 10 mg/L.