Expression of Rv2128 (A) and Rv0011c (B) in different constructs. Cells were harvested and then sonicated (W: whole cell). After centrifugation at 10,000 g for 20 min, the inclusion body fraction (IB) was collected. The supernatant was ultracentrifuged at 100,000 g for 45 min, and the supernatant (soluble fraction (S)) and pellet (membrane fraction (M)) were collected, respectively. The arrows indicate the overexpressed fusion proteins. Note that Rv2128 and Rv0011c could not be overexpressed without fusion partners, and there was no detectable expression for the KSI-Rv2128 fusion.