Figure 6

Immunocytochemical analysis for colocalization of CD147-intrabody. The transfected 293A cells were fixed and incubated with a mixture of biotinylated anti-human extracellular matrix metalloproteinase inducer (EMMPRIN) mAb and rabbit anti-HA mAb. Then, cells were stained with the mixture of Cy5-conjugated streptavidin and Cy3-conjugated anti-rabbit-IgG mAb. Nuclei were counterstained with DAPI (blue). Three-dimensional (3D) image of the transfected 293A cells was verified. A CD147 on transfected 293A cell stained with biotinylated anti-human EMMPRIN mAb (red), B scFv-M6-1B9 intrabody in transfected 293A cell stained with rabbit anti-HA mAb (white), C GFP positive in transfected 293A cell and D overlay. For 3D image of colocalization of CD147-intrabody see additional file.