Nucleotide sequence of cDNA and deduced amino acid sequence of the scFv-M6-1B9. The cDNA sequence encoding scFv-M6-1B9 was shown. Restriction endonuclease sites, His tags, and HA tag are indicated. The deduced amino acid sequence of scFv-M6-1B9 corresponding to the complementary determining regions (CDRs) in the variable regions of the L (red letters) and H (green letters) chains, which were identified by the Kabat numbering scheme, are indicated by gray boxes. Amino acids were numbered from the initiator methionine (M*). The amber stop codon was shown by an asterisk (*). The details of the CDRs region of scFv-M6-1B9 are shown as CDR1-VL C (24SV---LH35) W, CDR2-VL Y (51GT---AS 57) G, CDR3-VL C (90QQ---PL97) T, CDR1-VH S (153GF---MS162) W, CDR2-VH A (178IS---KG193) R, and CDR3-VH R (226FR---GAY231) W.