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Figure 3 | BMC Biotechnology

Figure 3

From: Viral vectors based on bidirectional cell-specific mammalian promoters and transcriptional amplification strategy for use in vitro and in vivo

Figure 3

GAL4p65 augments EGFP expression from synthetic biodirectional SYN and GfaABC1D promoters in the rat brain in vivo. A: Representative images from rats injected with LV-1 × SYN-EGFP (a), LV-mCMV/SYN-EGFP (b), LV-1 × GfaABC1D-EGFP (c) and LV-mCMV/GfaABC1D-EGFP (d). B: Assessing EGFP transgene expression level in vivo. (1): Relative EGFP fluorescence levels in rats transduced with LV-1 × SYN-EGFP or LV-mCMV/SYN-EGFP (n = 3). NIH ImageJ was used to quantitatively compare the relative EGFP fluorescence levels. Four sections surrounding the injection tract per rat were selected randomly and three fields from each section were used. (2): Relative EGFP fluorescence levels in rats transduced with LV-1 × GfaABC1D-EGFP or LV-mCMV/GfaABC1D-EGFP (n = 3). * p < 0.01, compared with LV-SYN-EGFP; ** p < 0.001, compared with LV-1 × GfaABC1D-EGFP. An unpaired t test was applied for comparisons between two groups. The differences were considered significant at p < 0.05. All values in the figures refer to mean ± SD.

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