Figure 4

Boxplot representation of the hybridization signal distributions for the individual mismatch types, arranged according to the median values (the 95% confidence bounds are depicted by the notch). Boxes indicate the interquartile range (from the 25th to 75th percentile) containing 50% of the data. Whiskers extend to a maximum value of 1.5 times the interquartile range from the boxes ends. differ significantly with a 95 percent confidence. Data processing: raw intensity data, solution-background correction, subtraction of the mean profile, normalization of the defect-type dependent deviations from the mean profile by division by the standard deviation of the defect profile (see Methods section). The mismatch types with the lowest hybridization signals are those (T·G, C·C, T·C, A·C, G·G) where C·G base pairs are affected by the mismatch defect. The only exception is A·G. The positive tails of this and other distributions seem to originate from stabilizing C·G base pairs next to the defect. $\Delta G_{37}^{\circ }$ (standard deviation assuming that the various MM nearest-neighbor types are equally distributed).