| Â | Dilution | Total | Nil | N per | Â |
Target | Factor | Rxns | Rxns | Aliquot | LDA-No |
CHS | 15,000 | 48 | 30 | 0.47 | 7,050 |
SE | 6,222 | 48 | 22 | 0.78 | 4,854 |
PHB | 6,222 | 48 | 28 | 0.54 | 3,354 |
EMF2 | 8,000 | 48 | 33 | 0.37 | 2,998 |
NPR1 | 3,000 | 48 | 26 | 0.61 | 1,839 |
ANT | 1,250 | 48 | 20 | 0.88 | 1,094 |
FLF | 500 | 24 | 9 | 0.98 | 490 |
HAP3C | 466 | 48 | 29 | 0.50 | 235 |
ABI3 | 250 | 48 | 28 | 0.54 | 135 |
AP3 | 67 | 48 | 19 | 0.93 | 62 |
Lec2 | 20 | 48 | 27 | 0.58 | 12 |
- Based upon the N0 values produced by LRE quantification, a sample of each reverse transcriptase reaction was diluted to a predicted 0.5–1.0 target molecules per aliquot for each of the eleven cDNA targets, and replicate amplification reactions were conducted to determine the frequency of aliquots lacking a target molecule (Nil Rxns). The number of target molecules (N) per aliquot was then calculated using equation 14 and the predicted number of target molecules in the reverse transcriptase reaction (LDA-No) was calculated by multiplying by the dilution factor (see text for additional details).
