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Table 2 Absolute quantification based upon conversion of Ct to F0 via E max and Ft.

From: A kinetic-based sigmoidal model for the polymerase chain reaction and its application to high-capacity absolute quantitative real-time PCR

Gain:

X8

X8

X2

X2

X1

  

OCF:

1092

1155

704

778

345

  

Enzyme:

QT 0XSG

QT 0.2XSG

DyNa 0.5XSG

DyNa 1.5XSG

FV 2.0XSG

LRE-No Average

CV (+/-)

Target

       

CHS K3K4

5,876

7,562

5,564

9,188

10,813

7,801

28.5%

SE K2K3

4,874

5,919

5,255

7,485

nd

5,883

19.6%

PHB K3K2

2,653

3,405

2,730

3,916

2,888

3,119

17.1%

EMF2 K2K3

2,256

3,271

2,960

2,821

nd

2,827

15.0%

NPR1 K1K4

2,039

2,070

1,634

1,892

2,369

2,001

13.4%

ANT K3K4

923

1,288

972

1,342

1,450

1,195

19.6%

FLF K3K4

442

651

489

665

nd

562

20.1%

HAP3C K3K4

148

195

255

285

nd

221

27.7%

ABI3 K3K4

65

108

139

139

46

100

42.6%

AP3 K3K4

32

54

40

39

26

38

27.6%

Lec2 K3K4

3

14

11

11

8

10

41.7%

      

Av. CV:

24.8%

  1. Ct values generated by the amplification profiles summarized in Figure 7 were converted to F0 using equation 6, and then converted to target molecule number via the OCF as used for LRE quantification (see text and additional data file 3 for additional details).
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BMC Biotechnology

ISSN: 1472-6750

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