Figure 10

An example of primer-specific baseline drift. An extreme example of baseline drift produced by lambda primer K30. (A) Amplification profiles of 100 femtograms of lambda gDNA with K23 and K30 primers (blue profile), K30 alone with no lambda gDNA (green profile) and K23 alone with no lambda gDNA (red profile) with no baseline subtraction. (B) The same profiles following baseline subtraction (F_b = average F_C of cycles 7–18). This illustraties the distortions that can be produced by the MxPro software used in this study, which attempts to correct for the baseline drift by modifying the F_C dataset. This can compromise, sometimes severely, the efficacy of LRE quantification, even in the absence of any apparent baseline drifting (data not shown)