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Table 1 Quantification of flow cytometric measurements (n = 5; 1.0*104 cells per sample).

From: Flow cytometric monitoring of influenza A virus infection in MDCK cells during vaccine production

 

uninfected

mock-infected

Infected

mean im

13.0

18.2

261.7

mean cMEF

4.19 *104

5.98 *104

9.02 *105

RSD of mean cMEF

± 8.45

± 2.46

± 1.66

c i

 

0.0

8.42 *10 5

  1. im = fluorescence intensity measured by flow cytometry
  2. cMEF = number of MEF per cell, according to calibration of fluorescence with Sphero
  3. FITC Calibration particles (eq. 1, fig. 5)
  4. ci = number of MEF per cell bound specifically to virus proteins in cells (eq. 2)