Table 1 Oligonucleotides for the synthesis of polA
From: PCR-based gene synthesis to produce recombinant proteins for crystallization
f22 | TTTGTTTAACTTTAAGAAGGAGATATACATATGCTCCGCAAGG |
f21 | ATATACATATGCTCCGCAAGGTCGAGGTACAAACCGTAGAGCCGGAGGACCGTGAAAGCT |
f20 | GGAGGACCGTGAAAGCTGGAACGACTTCCTGGAAAATCCACTCCTCAGCCTGTGGCTCGA |
f19 | TCAGCCTGTGGCTCGAGATGGACGGCGAAAACTATCATCGCGCAGAAATCATCGGTCTCG |
f18 | GCAGAAATCATCGGTCTCGCACTGTCTGATGGTGAGACCCATCTGTACGTCCCTTGGCGC |
f17 | GTACGTCCCTTGGCGCACTGCGCGTGACTGGGAGAACCTCCACCGTCTGCTCGCGGACGA |
f16 | TCTGCTCGCGGACGAGGAACGCAAGAAGATTGTTTATGATGGTAAACGTCTGCAAGTGGT |
f15 | GTAAACGTCTGCAAGTGGTGCTGAAGCGTCGTGGTCTGGAGGCTGGCGGTCTGGCTTTCG |
f14 | GGCGGTCTGGCTTTCGACGCGCTCCTGGCTTCTTATCTGCTGGACCCGTCCGAATCTGGC |
f13 | CCCGTCCGAATCTGGCCACTCTCTGTCCGACCTGGTACAGCGTAAGATGGATGGCTCTCT |
f12 | CGTAAGATGGATGGCTCTCTCCCACCTGATGAGGAGGTTTACGGTAAAGGTGCCAAACGC |
f11 | GGTAAAGGTGCCAAACGCCGCCTGCCAGGTGAGCGCGAACTCGCTGAACATCTCGCGCGT |
f10 | CTGAACATCTCGCGCGTAAAGCGGAAGCGCTCAAGCGCCTCTACCCACTGCTGAGCGAGG |
f9 | CCACTGCTGAGCGAGGAGATCCGTGAGGCCGGTATGGAGAGCCTGCTCTTTGAAATGGAA |
f8 | GCCTGCTCTTTGAAATGGAACTGCCACTGAGCCGTGTTCTGGCAGAGATGGAGCTGCATG |
f7 | CAGAGATGGAGCTGCATGGCGTACGTGTCGACCGCGACCGTCTCCTGGACCTGGGTGAAG |
f6 | CCTGGACCTGGGTGAAGAACTCAAGGAACAGGCAGAATCCCTCACGCGTCAAATCTACGA |
f5 | TCACGCGTCAAATCTACGAACTGGCGGGTACCGAATTCAACATTAACTCTCCGAAACAGC |
f4 | ACATTAACTCTCCGAAACAGCTCGCCGAGATTCTCTACGACAAACTGGGTCTGCCGGTTC |
f3 | CTGGGTCTGCCGGTTCTCAAAAAGACTAAAACGGGCTATTCTACCTCTGCGGACGTTCTG |
f2 | CCTCTGCGGACGTTCTGGAGAAACTGGCACCGCAACATGAAATCGTTGAAAAAATCCTGC |
f1 | GAAATCGTTGAAAAAATCCTGCACTACCGCCAAATCATGAAACTGATCTCCACCTATGTC |
r1 | TTTACCGGATTCCGGGTCGATTTCCTTCAGGAGGCCCTCGACATAGGTGGAGATCAGTTT |
r2 | GCGGCCAGTTGCAGTGATAGTCTGGTTGAAACGCGTGTGAATTTTACCGGATTCCGGGTC |
r3 | CAGGCGAATCGGGATATTTTGCAGGTTCGGCTCGGTAGAGCTGAGGCGGCCAGTTGCAGT |
r4 | CAGACGGAACGAATACCTGACGGATGCGACGACCTTCCTCCAGGCGAATCGGGATATTTT |
r5 | AATCTGAGAGTAATCTGCGCTCAGGATCTGCCAACCCGGTTCAGACGGAACGAATACCTG |
r6 | GAGACTCGTCGCCAGACAGATGAGCCAGTACGCGGAGCTCAATCTGAGAGTAATCTGCGC |
r7 | GTCTTAGTATGAATGTCCATGTCTTCGCTAAAAGCCTGTTTGAGAGACTCGTCGCCAGAC |
r8 | AACTTCATCTTCAGGGACACCGAACACATCCATAGCGGTCTTAGTATGAATGTCCATGTC |
r9 | CGAAATTAACCGCCTTTGCTTGACGACGCATGAGAGAGGTAACTTCATCTTCAGGGACAC |
r10 | GTTTTGGGACAGGCCGTAATCAGAAATGCCGTAGATGATACCGAAATTAACCGCCTTTGC |
r11 | AACGTTCAATGAATTGCGCCGCTACTTTACGTGGGATGTTGAGGTTTTGGGACAGGCCGT |
r12 | ACGATCCATGTATTCTTTAACACCAGGGTAAGATTGGAAGTAACGTTCAATGAATTGCGC |
r13 | TGGTAACGTAACCGTCTTTACGGGCTTGCTCTACAACACGATCCATGTATTCTTTAACAC |
r14 | AGCGAATTTCCGGGAGGTAGCGACGGCGGTTGAGCATGGTGGTAACGTAACCGTCTTTAC |
r15 | ATCGCCGTACGTTCCGCGAAAGTGCGGCGATTGTAGTTACGAGAGCGAATTTCCGGGAGG |
r16 | CGGTTTTGATAATATCTGCCGCAGAACCCTGGATCGGGGTGTTCATCGCCGTACGTTCCG |
r17 | CGCGGCGACGTTTGATCTCACGATGCAGGCGTACCATGGCGGTTTTGATAATATCTGCCG |
r18 | CAAAAATGAGTTCGTCGTGAACTTGGAGCAGCATACGAGACTTAACGCGGCGACGTTTGA |
r19 | CCAGATTTTTCATTTCTTCGAGTTCCTCTTCTGGAACTTCAAAAATGAGTTCGTCGTGAA |
r20 | ACGGAGAGCGGGACCGCTTGTTCCATAACCGTACGAACCAGATTTTTCATTTCTTCGAGT |
r21 | GCTTCATACCAAGTCTGACCGGTGTGGATATCAACTTTGAGCGGCACGGAGAGCGGGACC |
r22 | CTTCCTTTCGGGCTTTGTTAGCAGCCGGATCCTTATTTGGCTTCATACCAAGTCTGACC |