Table 3 Steady-state kinetic parameters for the cleavage of various nucleic acids by GBSV1-NSN and other nucleases.
From: Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1
Enzyme | Substrate | K m | k cat | k cat / Km | Reference | |
|---|---|---|---|---|---|---|
ug·ml-1 | μM | s-1 | uM-1·s-1 | |||
GBSV1 -NSN | herring sperm DNA | 75 | 231 | 1278 | 5.5 | this work |
heat-denatured DNA | 20 | 61 | 241 | 4.0 | this work | |
Baker's yeast RNA | 30 | 92 | 300 | 3.3 | this work | |
NucA | herring sperm DNA | 53 | 164 | 1921 | 11.7 | [24] |
scDNA | 82 | 253 | 2111 | 8.3 | [24] | |
E. coli tRNALeu | 83 | 257 | 2255 | 8.7 | [24] | |
heat-denatured DNA | 41 | 126 | 2170 | 17.2 | [24] | |
SNase | heat-denatured DNA | 15 | 46 | 765 | 16.6 | [24] |
SMNase | herring sperm DNA | 20 | 61 | 980 | 16.1 | [24] |
DNase A | herring sperm DNA | 20 | 61 | 330 | 5.4 | [3] |