Table 2 Effects of metal chelators, thiol reagents and detergents on the GBSV1-NSN nuclease activity. The concentrations of additives represented their concentrations in the incubation solutions with the nuclease and in the final nuclease reactions. The error limits shown were the standard deviations.
From: Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1
Reagents | Concentrations in incubation solution/in final nuclease reaction | Relative activity (%) |
|---|---|---|
None | 0 | 100 |
EDTA | 1 mM/0.5 mM | 96.9 ± 3.2 |
10 mM/5 mM | 85.1 ± 2.8 | |
Citrate | 1 mM/0.5 mM | 94.2 ± 2.9 |
10 mM/5 mM | 66.8 ± 2.2 | |
DTT | 1 mM/0.5 mM | 52.8 ± 1.6 |
10 mM/5 mM | 34.1 ± 1.2 | |
β-ME | 1 mM/0.5 mM | 89.5 ± 2.5 |
10 mM/5 mM | 87.8 ± 2.7 | |
Urea | 1 M/0.5 M | 63.9 ± 2.1 |
Guanidine hydrochloride | 0.6 M/0.3 M | 51.0 ± 1.8 |
SDS | 0.1 %/0.05% | 61.8 ± 2.0 |
1 %/0.5% | 73.4 ± 2.2 | |
Tween-20 | 0.1 %/0.05% | 138.9 ± 3.7 |
1 %/0.5% | 141.6 ± 3.9 | |
TritonX-100 | 0.1 %/0.05% | 124.5 ± 3.2 |
1 %/0.5% | 133.7 ± 3.6 | |
Chaps | 0.1 %/0.05% | 126.2 ± 2.9 |
1 %/0.5% | 140.6 ± 3.2 |