Table 1 Effects of metal ions on the GBSV1-NSN enzymatic activity. The concentrations of metal ions represented their concentrations in the incubation solutions with the nuclease and in the final nuclease reactions. The error limits shown were the standard deviations.
From: Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1
Metal ions | Relative activity (%) | ||
|---|---|---|---|
Concentrations in incubation solution/in final nuclease reaction | 0.1 mM/0.05 mM | 1 mM/0.5 mM | 10 mM/5 mM |
None | 100 | 100 | 100 |
Mn2+ | 92.4 ± 2.9 | 102.5 ± 3.1 | 130.8 ± 4.1 |
Zn2+ | 101.4 ± 3.1 | 114.7 ± 3.0 | 118.4 ± 2.9 |
Sr2+ | 96.8 ± 2.8 | 101.1 ± 2.9 | 102.3 ± 2.1 |
Ba2+ | 98.0 ± 3.1 | 97.8 ± 2.8 | 92.4 ± 2.9 |
Mg2+ | 93.3 ± 1.9 | 92.9 ± 2.2 | 87.8 ± 2.6 |
Ca2+ | 98.3 ± 2.2 | 94.6 ± 2.4 | 92.2 ± 2.8 |
Ni2+ | 93.2 ± 1.0 | 89.5 ± 1.5 | 86.4 ± 2.2 |
Li+ | 93.6 ± 3.0 | 92.3 ± 2.8 | 91.0 ± 2.4 |
Na+ | 92.5 ± 2.9 | 81.9 ± 2.7 | 80.8 ± 1.7 |
K+ | 95.1 ± 1.9 | 87.5 ± 2.1 | 83.9 ± 2.6 |
Cs+ | 98.7 ± 3.2 | 95.1 ± 1.6 | 89.8 ± 2.5 |
Cu2+ | 74.2 ± 2.3 | 55.5 ± 1.4 | 3.6 ± 0.1 |
Fe3+ | 67.7 ± 1.5 | 22.3 ± 0.8 | 0.7 ± 0.1 |