Figure 6From: Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1The Lineweaver-Burke plot. The kinetic parameters Km and Vmax were estimated by linear regression from Lineweaver-Burk plots. The nucleic acid concentrations were varied in the range of 0–100 μg/ml. All nuclease activity assays were conducted at 60°C and pH 7.5. Each point represented the mean of triplicate assays and the error bars represented the standard deviations.Back to article page