Figure 3
From: Characterization of a novel non-specific nuclease from thermophilic bacteriophage GBSV1
Digestion of various nucleic acids by the purified GBSV1-NSN protein. The purified GST protein was used as negative control. The commercial RNase A (A) or DNase I (B, C and D) was also included as positive control. The protein solutions were shown on the top and the nucleic acids were indicated on the right. 1 μg of nucleic acids were respectively incubated with 1.5 μg of the purified GBSV1-NSN protein in 20 μl of reaction buffer at 37°C for six hours.