Figure 6From: In vivosite-specific biotinylation of proteins within the secretory pathway using a single vector systemBiotinylation dependence on BirA localization. Western blot gel retardation assay on cells co-transfected with plasmid constructs encoding secretory (sec) or cytosolic (cyt) BirA, and scFv1E10 (A), t-sIgE (B) or NSP5 (C). Where indicated, supernatants (dialyzed) or cell extracts were reacted with streptavidin.Back to article page