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Figure 3 | BMC Biotechnology

Figure 3

From: Simultaneous monitoring of independent gene expression patterns in two types of cocultured fibroblasts with different color-emitting luciferases

Figure 3

Analysis of the circadian phases of Bmal1–GR and Bmal1–RED cells cocultured with direct contact. The bioluminescence activities of Bmal1–GR (A), Bmal1–RED (B), and cocultures of these cells (C) were measured. The luciferase activities are shown on the Y axis and the time after starting measurement of the bioluminescence is shown on the X axis. These cultures showed clear circadian rhythms. D: Average peak times after the start of DEX treatment of Bmal1–GR cells in monoculture (filled diamonds), in coculture (open diamonds), Bmal1–RED cells in monoculture (filled circles) and in coculture (open circles). The number of samples is shown under the symbols. The bars show the SEM of the data. The phases of each cell line gradually became separated in both cocultures and monocultures. E: Amplitude-damping rates of Bmal1–GR and Bmal1–RED cells in monoculture and coculture are shown. There were no significant differences in damping rates between cell lines or between monocultures and cocultures (P > 0.05, Mann-Whitney U test).

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