Culture medium for expression | Promoter/Inducer | Location of the target gene | DCW/La/Induction time | |
---|---|---|---|---|
EBY100-pLHJ026 | YNB-CAA containing glucose or galactose | GAL1/galactose | Cloned in the episomal plasmid | 5.6 g/2 days |
KM71-pLHJ047 | BMGY, BMMY | AOX1/methanol | Integrated into genome | 26.3 g/4 days |
KM71-pLHJ048 | BMGY, BMMY | AOX1/methanol | Integrated into genome | 25.6 g/4 days |