Figure 5From: Efficient display of active lipase LipB52 with a Pichia pastoris cell surface display system and comparison with the LipB52 displayed on Saccharomyces cerevisiae cell surfaceThe effect of temperature on stability of the displayed lipases. The lipase LipB52 displayed on Pichia pastoris KM71-pLHJ047 and KM71-pLHJ048 cell surface retained over 90% activity after incubation at 60°C for 2 hours at pH 7.0, and they can still retained about 80% activity after incubation for 3 hours. The lipase LipB52 displayed on Saccharomyces cerevisiae EBY100-pLHJ026 retained near 90% activity after incubation at 60°C for 2 hours at pH 7.0 and it can only retained about 70% activity after incubation for 3 hours.Back to article page