Figure 5

The effect of temperature on stability of the displayed lipases. The lipase LipB52 displayed on Pichia pastoris KM71-pLHJ047 and KM71-pLHJ048 cell surface retained over 90% activity after incubation at 60°C for 2 hours at pH 7.0, and they can still retained about 80% activity after incubation for 3 hours. The lipase LipB52 displayed on Saccharomyces cerevisiae EBY100-pLHJ026 retained near 90% activity after incubation at 60°C for 2 hours at pH 7.0 and it can only retained about 70% activity after incubation for 3 hours.