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Figure 1 | BMC Biotechnology

Figure 1

From: Efficient display of active lipase LipB52 with a Pichia pastoris cell surface display system and comparison with the LipB52 displayed on Saccharomyces cerevisiae cell surface

Figure 1

The structure of the expression plasmid pLHJ047 and p LHJ048. 5'AOX1, alcohol oxidase 1 promoter; S, α-factor signal sequence for secretion in Pichia; FLO, FLO gene coding for FLO protein from Saccharomyces cerevisiae; lipB52, lipase gene lipB52 cloned in frame and downstream of the FLO sequence; 3'AOX1(TT), transcriptional terminator from pichia pastoris AOX1gene; HIS4, pichia wild-type gene coding for histidinol dehydrogenase and used to complement Pichia his4- strains; kanr, Kanamycin resistance gene from Tn903 which confers resistance to G418 in Pichia; 3'AOX1, Sequences from the AOX1 gene that are further 3' to the TT sequences; CoE1, E. coli origin of replication; Ampr, Ampicillin resistance gene.

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BMC Biotechnology

ISSN: 1472-6750

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