Figure 3

Morphometrical analysis of nervous regenerated area. (A) Scheme of the arbitrary coordinates representing the distance from the proximal side as a percentage of the total conduit length adopted to localize the estimated nervous regenerated cross-section. Cross-sectional area measurements positive to Bielschowsky reaction (B), to anti β-tubulin antibody (C) and to anti NF200 antibody (D) stainings are comparable and reveal regeneration throughout the conduit lengths. Images of regenerated area positive for the aforementioned stainings detected at 75% of the conduit length are shown on the right side of each graphic. Values for the regenerated area decrease at the middle length of the conduit. Area quantification is indicated in square mm. Error bars represent standard deviation (N = 12).