Figure 3

Growth rate of 14-day old preantral follicles derived from vitrified mouse preantral follicles and vitrified whole ovaries. The granulosa cells outgrowth rate of 14-day old preantral follicles (diamond, unfrozen preantral follicle, n = 40; square, vitrification in 2 μl volume, n = 40; triangle, vitrification in 6 μl volume, n = 38), and 14-day old preantral follicles derived from frozen-thawed transplanted ovaries of newborn mice (circle, vitrified in 6 μl volume, n = 31). No significant difference was found in the diameter of the granulosa cell outgrowth of follicles after 8 day of culture between in 2 μl and 6 μl vitrification groups. The comparison of granulosa outgrowth was not demonstrated at day 10 and day 12, because the overgrowth of the antral cavity and hatched oocyte from antral cavity were frequently found in preantral follicles from 14-day old mice.