Figure 4

Real-time RT-PCR of patatin hpRNAi lines. Real-time RT-PCR was performed on RNA extracted from 10 independent hpRNAi lines using primers designed against the patatin gene (pat3-k1). WT is a wild-type positive control. Lanes 1 to 10 are ten independent patatin hpRNAi lines. 18S rRNA was amplified as a control for equal amounts of RNA templates. Real-time RT-PCR results show relative fold changes of pat3-k1 transcript levels in hpRNAi lines in comparison with WT. The error bars indicate mean ± SD. Mean and SD were derived by two independent experiments. pat3-k1 transcript levels were significantly reduced in most hpRNAi lines, compared to the WT control.