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Figure 2 | BMC Biotechnology

Figure 2

From: Development of patatin knockdown potato tubers using RNA interference (RNAi) technology, for the production of human-therapeutic glycoproteins

Figure 2

Characterization of T-DNA insertions in patatin hpRNAi lines. (A) PCR amplification of npt II gene in ten patatin hpRNAi lines. The PCR results confirmed that lanes 1 to 10 are patatin hpRNAi lines. Lane PC (DNA of hpRNAi vector was used as a template for PCR) or lane WT (genomic DNA of WT plants was used as a template for PCR) is the positive or negative control, respectively. Lanes 1 to 10 are patatin hpRNAi potato lines. (B) DNA gel blot of wild-type and seven patatin hpRNAi lines. Genomic DNA was digested with XbaI and probed with npt II gene probe. Lines 4, 7, 8, and 10 each have one T-DNA insertion.

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