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Figure 1 | BMC Biotechnology

Figure 1

From: Development of patatin knockdown potato tubers using RNA interference (RNAi) technology, for the production of human-therapeutic glycoproteins

Figure 1

Map of patatin-hpRNAi binary vector. A single PCR product (nucleotides 188–798) from the target gene pat3-k1 was cloned into pKANNIBAL vector in an inverted-repeat orientation (sense-oriented copies in XhoI – KpnI site; anti-sense oriented copies in ClaI – XbaI site). pKANNIBAL vector was digested with NotI enzyme and then NotI fragments were cloned into pART27 binary vector.

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BMC Biotechnology

ISSN: 1472-6750

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