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Figure 7 | BMC Biotechnology

Figure 7

From: Nanopolymers improve delivery of exon skipping oligonucleotides and concomitant dystrophin expression in skeletal muscle of mdx mice

Figure 7

nNOS expression in mdx mice after treatment with PEG-PEI-ESO. Membrane-associated nNOS expression is upregulated in PEG-PEI-ESO treated mdx muscles in regions highly positive for dystrophin expression. TA muscles were given 10 twice-weekly intramuscular injections of 5 μg of ESO complexed with the NG-PEI2K(PEG550)10 copolymer, and harvested 6 wks after the first injection. (A-B) Images of serial whole transverse sections immunolabeled for dystrophin (A) and nNOS (B) show the correlation in ESO-mediated upregulation of these two membrane-associated proteins. (C) Higher magnification images of specific regions from panels A and B show clearly the concomitant (co-localized) upregulation of both dystrophin and nNOS in the same set of fibers of the polyplex-injected muscles. As expected nNOS expression in untreated mdx controls was negligible.

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