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Figure 4 | BMC Biotechnology

Figure 4

From: Nanopolymers improve delivery of exon skipping oligonucleotides and concomitant dystrophin expression in skeletal muscle of mdx mice

Figure 4

Quantitative analysis of dose-response profile of dystrophin expression after intramuscular injections of CG-PEI2K(PEG5K) 10 -ESO polyplexes. Muscles were analyzed following 3 weekly intramuscular injections of 1, 5, 10, or 20 μg of ESO (3, 15, 30, and 60 μg total) complexed with CG-PEI2K(PEG5K)10 copolymers, and harvested 3 wks after the first injection. (A) Number of dystrophin-positive fibers for each dosage was obtained from whole transverse sections that were immunolabeled for dystrophin. Number of dystrophin-positive fibers was significantly lower for the 1 μg injections than for all other doses (P < 0.05, N = 4 muscles per group). (B) Quantification of dystrophin expression as a percentage of age-matched normal mice for each of the 4 polyplex dosages based on densitometry of western blots (not shown). Samples were prepared from thick (60 μm) transverse cryosections taken adjacent to segments used for the fiber counts. Each increase in dosage resulted in significantly greater level of dystrophin expression, except between the 30 μg and 60 μg dosages which reached a plateau.

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