Table 1 ELISA competition studies on RhoAQ63L coated plate. ScFvC1-N1N2 (0.4 μM) is competed by increasing concentrations of GST-RBD, GDP bound RhoA, GTP bound Cdc 42, GTP bound Rac 1. KD of GST-RBD was also determined by surface plasmon resonance on GST-RhoAQ63L.
From: Identification of a GTP-bound Rho specific scFv molecular sensor by phage display selection
| Â | GTP-RhoA | GDP-RhoA | GTP-Cdc42 | GTP-Rac1 |
|---|---|---|---|---|
GST-RBD KD (μM) | 0.2 ± 0.2 | > 50 | > 50 | > 50 |
ScFvC1N1N2 KD (μM) | 3 ± 1 | > 50 | > 50 | > 50 |