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Table 1 ELISA competition studies on RhoAQ63L coated plate. ScFvC1-N1N2 (0.4 μM) is competed by increasing concentrations of GST-RBD, GDP bound RhoA, GTP bound Cdc 42, GTP bound Rac 1. KD of GST-RBD was also determined by surface plasmon resonance on GST-RhoAQ63L.

From: Identification of a GTP-bound Rho specific scFv molecular sensor by phage display selection

  GTP-RhoA GDP-RhoA GTP-Cdc42 GTP-Rac1
GST-RBD KD (μM) 0.2 ± 0.2 > 50 > 50 > 50
ScFvC1N1N2 KD (μM) 3 ± 1 > 50 > 50 > 50