Skip to main content

Table 3 Primers for the synthesis of in vitro RNA standards. The T7 promoter sequence CTAATACGACTCACTATAGGGAGA was added to the 5'-end of all primer 1 sequences.

From: Improved production of human type II procollagen in the yeast Pichia pastoris in shake flasks by a wireless-controlled fed-batch system

Probe name

Sequence (5'-3')

Position

Human collagen II

  

Primer 1

GCCAGGTAGAGAAGGATCAC

2892

Primer 2

CTGCTGCCTCGTCCAAGTAA

4006c

C-P4Hα subunit

  

Primer 1

GGCAGAAGAGGACAAGTTAG

141

Primer 2

ACAGGCTGCATGCCGTGTA

1509c

C-P4H PDIsubunit

  

Primer 1

AAGTACCTGCTGGTGGAGTT

124

Primer 2

TCATCGTCTTCCTCCATGTC

1499c

AOX1 gene

  

Primer 1

AGCAGGTGAGAACAACCTCAAC

583

Primer 2

AAGGTCCACCGTAGGCATTAGA

1988c

EF3 gene

  

Primer 1

CGAGTTCGTCAAGCGTGTTC

546

Primer 2

CTCGTCCAGGACGATCAAGT

1434