Table 2 Probes used in RNA sandwich hybridization. The capture probes were labeled with biotin and the detection probes contained a digoxigenin (DIG) tail.
Probe name | Sequence (5'-3') | Position/Modification |
|---|---|---|
human collagen II | ||
Helper probe | GCTCCTGGTGATCCTTCTCTA | 2918c |
Capture probe | CTCTACCTGGAGGACCATCG | 2938c [5' biotin] |
Detection probe | CTTTAACTCCGGCAGCACCAT | 2959c [3' DIG tail] |
C-P4Hα subunit | ||
Helper probe | TCTGCTTCTGTATAGGCCAC | 536c |
Capture probe | TGTTCCATCCACAGTTCCGT | 566c [5' biotin] |
Detection probe | ATCTCGCCTTCATCCAGTTG | 596c [3' DIG tail] |
C-P4H PDI/β subunit | ||
Helper probe | CTTGAAGCTCTCGGCTGCTG | 849c |
Capture probe | TGGTCGCTGTCGATGAAGATG | 884c [5' biotin] |
Detection probe | TCGAGGATGCGCTGGTTGTC | 908c [3' DIG tail] |
AOX1 gene | ||
Helper probe | GACTCGTACTGAGGCTTG | 1440c |
Capture probe | GAATCTCAGCATCACCAC | 1475c [5' biotin] |
Detection probe | ACCATTGGCGTACCATTG | 1510c [3' DIG tail] |
EF3 gene | ||
Helper probe | GAATTGACCGGAAGCCAA | 1269c |
Capture probe | AGCCTCCTTCATATCGAC | 1251c [5' biotin] |
Detection probe | CTCAGCAACCATCTTGGA | 1233c [3' DIG tail] |