Skip to main content
Figure 4 | BMC Biotechnology

Figure 4

From: A set of ligation-independent in vitro translation vectors for eukaryotic protein production

Figure 4

In vitro kinase activity of translated versus overexpressed AtMPK6. MBP phosphorylation activity of E. coli overexpressed (E) and translated (T) AtMPK6 was detected by autoradiography (A). Coomassie Blue staining shows equal loading of MBP (C). AtMPK6 was either produced in E. coli or translated by bilayer method, and 100 ng of purified kinase was used in kinase reactions.

Back to article page