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Figure 2 | BMC Biotechnology

Figure 2

From: A set of ligation-independent in vitro translation vectors for eukaryotic protein production

Figure 2

SDS-PAGE analysis of in vitro translated proteins. A. CEFC translation reactions with pEU3-NII backbone vector constructs: Molecular weight marker (M), wheat germ extract control (C), 1 μl out of 50 μl in vitro translation reaction mixture with pEU3-NII-HLIC (H) or pEU3-NII-GLIC (G) vector. B. Bilayer translation reactions with pEU-E01 backbone vector constructs: Molecular weight marker (M), wheat germ extract (C), 5 μl out of 225 μl in vitro translation reaction mixture with pEU-E01-HLIC (H) or pEU-E01-GLIC (G) vector. Proteins present in the translation mixtures were separated on 12% SDS-PAGE gel and detected by Coomassie Blue staining. Vector encoded-kinases are indicated by asterisks.

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